The Bionic Bioreactor Company
BioThrust builds the only bioreactor worldwide that achieves high gas transfer rates without high shear forces and foam
Introducing ComfyCell
A whole new approach to stirred-tank bioreactors for 3D cell therapy manufacturing.
Powered by our unique Membrane Stirrer technology
The BioThrust Membrane Stirrer aerates the cell culture bubble-free & minimizes shear forces with gentle mixing.
Broad Range
Of Applications
Cell Types
More than 258 million CHO cells per mL without foam!
Over 9 days, the ComfyCell (leveraging an ATF system) achieved an impressive cell density of 258E6 CHO cells per mL at 2L scale with a PCV of ~50%. No foam was observed at all!
In a separate 10L fed-batch run compared to a thoroughly optimized conventionally sparged process, the Membrane Stirrer achieved the same top-notch cell density, but without the need to add any oxygen to the input gas or use anti-foam.
Cell Types
Up to 400% increased product yield with perfusion
In a 2L ATF perfusion process, reaching 258E6 CHO cells per mL, the ComfyCell achieved a 60g titer through the ATF membrane. This 30g/L product yield exceeded the STR control by 4x, which usually achieves 7g/L. The final product yield was diluted over 50L.
In a 10L fed-batch benchmark against an optimized conventional STR, the ComfyCell achieved +40% product conversion and didn't require any additional oxygen to be added to the process gas
Cell Types
Over 93 million cells per mL in an ATF perfusion run
The ComfyCell achieved over 93E6 cells per mL during a 13-day process with HEK293 cells at 2L scale, maintaining >90% average cell viability. No foaming was observed since sparging is not required!
Cell Types
Experiments & evaluation ongoing - Contact us!
Stay tuned for further updates & publications or contact us if you want to collaborate! We are aiming to confirm that our foam free high-density HEK process & in-situ perfusion system can achieve superior AAV yield.
Cell Types
More than 19.1 million iPSCs per mL with 93% viability!
Human iPSC were expanded as spheroids with remarkable success, reaching an impressive 19E6 cells per mL leveraging simple dip tube perfusion designed by BioThrust! Viability and aggregate characteristics were excellent, paving the way for therapeutic applications at high efficiency.
Human iPSCs were also easily expanded on carriers, reaching an expansion factor of 65! Benchmarked against a conventional bioreactor, our system delivered outstanding performance with a threefold increase in total cell numbers, surpassing 1.6E9 total cells in 250mL!
Cell Types
Successful completion of first human bm-MSC run
Using RoosterBio's human bm-MSC cell line, medium and supplement we were able to confirm expansion with excellent average viability.
Stay tuned for further updates & publications as we optimize the processor contact us to collaborate!
Cell Types
6.4 million EVs per mL yielded from human bm-MSCs!
The ComfyCell achieved an impressive EV density of 6.4E9 particles/mL from human bm-MSCs in our 300mL Single-Use Bioreactor. This was 5 times more than from the 2D control and resulted in a batch of 1.9E12 EVs
Also, with THP-1 cells, the 300mL ComfyCell SUB achieved a VCD of 2.19Mn cells/mL (outperforming static culture by 50%) and a EV density of 4.41Bn particles/mL (outperforming static culture by 83%) - with results confirming stable production of similar EVs.
Cell Types
Impressive >327-fold expansion of iNK cells at 2L
iNK cells were expanded in our 300mL SUB and our 2L reusable system, proving the simplicity of upscaling with the ComfyCell. An amazing >327-fold expansion of iNKs (>1000-fold from thaw) while maintaining excellent killing performance confirms the viability of the ComfyCell for commercial iNK production as we reached >3E9 cells in a batch.
NK-92 cells and primary NKs were also successfully expanded at 2L and 300mL respectively - with excellent quality and no foaming!
Cell Types
Achieved conventional STR cell density 2 days faster!
In a fed-batch POC with CD4+/CD8+ T-Cells isolated from a healthy donor, the ComfyCell achieved a 60-fold expansion in 5 days, yielding 7.2E6 cells/mL in our 300mL Single-Use Vessel. A conventional 250mL STR reached this density in 7 days - 2 days slower!
Stay tuned for further updates & publications or contact us to collaborate!
Cell Types
Up to 3mm large organoids cultivated over 35 days in 2L
Compared to conventional runs, our Membrane Stirrer achieved the closest to in-vivo results for iPSC-derived lung organoids our academic partner ever measured, producing up to 3mm large organoids and 100% living cells inside the structure after a process period of 35 days!
Cell Types
More than +200 % increased process yield
Compared to a conventional stirred-tank system, our Membrane Stirrer doubled the achieved cell density and increased production volume by further 30% due to foam avoidance.
Cell Types
Experiments & evaluation ongoing - Contact us!
Stay tuned for further updates & publications or contact us to collaborate!
Cell Types
More than 3-fold increase of rhamnolipid production and 65% larger batch volume
The MemStir demonstrated a space-time yield (STY) of up to 118mgRL L-1h-1, ranking it high in industrial rhamnolipid (RL) production with both conventional and complex fermentation methods. All without any use of anti-foam due to no bubbles!
Cell Types
Experiments & evaluation ongoing - Contact us!
Stay tuned for further updates & publications or contact us to collaborate!
Process Modalities
Suspension Cells
Leverage the homogeneous, gentle mixing and gas supply of our Membrane Stirrer for a physiological cultivation of your suspension cells (e.g., NK Cells).
Process Modalities
Adherent Cells
Grow your adherent cells (e.g., MSCs) on microcarriers that are gently kept in motion by the Membrane Stirrer.
Process Modalities
Spheroids
Manage the size and growth of your three dimensional cell aggregates and spheroids (e.g., iPSCs) with the precisely controllable shear force induced by the Membrane Stirrer.
Process Modalities
Differentiation Processes
Perform the whole process from cell expansion to differentiation in our ComfyCell Vessel. The currently in development in-situ perfusion system will enable a complete media exchange and the automatic supply of specific substances to trigger differentiation.
Process Modalities
ATF/TFF Perfusion
Leverage industry standard ATF and TFF perfusion systems for continuous supply of nutrients and media exchange through one of the PG13.5 ports. Ideal for ultra high cell density and maximizing yield.
Process Modalities
In-situ Perfusion
Leverage our proprietary in-situ perfusion system for continuous supply of nutrients and media exchange through a cell retention filter across the entire vessel bottom. Ideal for cost-efficient medium densities and maximizing product extraction.
Discover Our Application Studies
Our Advocates
Experience The Comfycell Platform
Developed to meet your specific need, from reusable to single-use, from lab to commercial scale.
Experience Comfycell for yourself.
Convince yourself of the new generation of bioreactors - short delivery times, attractive financing models, and process experts by your side make getting started as easy as possible for you.
